《植物生理学报》 2014, 50(1): 61-67

蒙古沙冬青对灰斑古毒蛾重复取食响应的代谢组学分析

孙敬茹^1,2, 张笑^1,2, 沈应柏^1,2,*, 杨海军^3,*

¹北京林业大学生物科学与技术学院, 北京100083; ²北京林业大学林木、花卉遗传育种教育部重点实验室, 北京100083; ³清华大学化学系微量分析测试方法与仪器研制北京市重点实验室, 北京100084

通信作者：沈应柏；E-mail: ybshen@bjfu.edu.cn, cyhj@tsinghua.edu.cn；Tel: 010-62336563, 010-62788971

摘　要：

植物对同种昆虫重复取食做出更有效的响应是植物适应自然界虫害周期性爆发的重要策略。为了研究蒙古沙冬青对其主要害虫灰斑古毒蛾幼虫重复取食响应的代谢基础, 本实验采用基于核磁共振(NMR)的代谢组学技术, 分析了遭受同一年不同代灰斑古毒蛾幼虫取食的蒙古沙冬青叶片和对照组幼苗叶片的代谢差异。结果表明, 仅遭受第一代幼虫取食叶片在第二代幼虫期与对照之间代谢物存在较小差异, 而仅遭受第二代幼虫取食叶片与遭受两代幼虫取食叶片代谢差异显著, 仅遭受第一代与仅遭受第二代幼虫取食的植株相邻分枝叶片差异显著。另外, 只有仅遭受第二代幼虫取食组幼苗表现出处理叶片与相邻分枝叶片的显著代谢差异, 并且这种代谢差异为总体代谢的变化, 而并未表现为特定代谢物含量变化。由此可见, 蒙古沙冬青能在代谢水平以很小的代谢变化来保存昆虫取食信息, 在遭受同种昆虫取食后, 代谢差异被放大, 而且这种信息保存在遭受取食叶片中表现更明显。

关键词：蒙古沙冬青; 灰斑古毒蛾; 同种昆虫; 代谢组学

收稿：2013-09-16   修定：2013-11-08

资助：国家自然科学基金(31270655和30871727)和高等学校博士学科点专项科研基金(20090014110014)

Metabolic Effects of Early-Season Herbivores on the Response to Conspecific Attack in Later-Season in Ammopiptanthus mongolicus (Maxim.) Cheng f

SUN Jing-Ru^1,2, ZHANG Xiao^1,2, SHEN Ying-Bai^1,2,*, YANG Hai-Jun^3,*

¹College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China; ²Key Laboratory of Genetics and Breeding in Forest Tree and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 100083, China; ³Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China

Corresponding author: SHEN Ying-Bai; E-mail: ybshen@bjfu.edu.cn, cyhj@tsinghua.edu.cn; Tel: 010-62336563, 010-62788971

Abstract:

More effective response to attack by subsequent conspecific herbivores in plants may be important strategies to adapt to periodic outbreaks of some insects, however, the mechanism for plants to respond to this kind of attack remains unclear. In order to investigate the metabolic basis of Ammopiptanthus mongolicus for periodic attack of Orgyia ericae, seedlings infested with different generations of O. ericae larvae that lived in early-season and later-season of a year were analyzed with nuclear magnetic resonance (NMR)-based metabolic technology. Results presented in this experiment indicated that leaves attacked by early-season herbivores showed a minor difference in metabolic profiling at the later season compared with control, whereas leaves attacked by later-season herbivores showed significant difference compared with leaves infested with both early-season and later-season herbivores. For leaves on sister branches, only leaves from seedlings infested with early-season and later-season herbivores showed significant difference. Additionally, significant difference between local and leaves on sister branches only existed in seedlings attacked by later-season herbivores. Therefore, A. mongolicus save the information of O. ericae larvae infestation with minor metabolic changes mainly in local leaves, which would be amplified when attacked by conspecifics.

Key words: Ammopiptanthus mongolicus; Orgyia ericae; conspecifics; metabolomics